Can you launch that item & layer through Application Studio? I had a similar issue with my testing except that my live is 2024.1 and in it you assign a Menu ID and Layer but in 2025.1 it has changed to giving it the Program Name and Layer, updating mine from Menu ID to Program solved the problem for me. It looks like you already have the program in there so the next thing I would check is to make sure you can access it through Application Studio and that the layer is published.